Investigating the reactivities of a polyketide synthase module through fluorescent click chemistry.

Modular polyketide synthases: Investigating intermodular communication using 6 deoxyerythronolide B synthase module 2.

A novel variant of 6-deoxyerythronolide B synthase (DEBS) module 2 was constructed to explore the balance between protein-protein-mediated intermodular channeling and intrinsic substrate specificity within DEBS. This construct, termed (N3)Mod2+TE, was co-incubated with a complementary, donor form of the same module, (N5)Mod2(C2), as well as with a mutant of (N5)Mod2(C2) with an inactive ketosyn...

Tylosin polyketide synthase module 3: stereospecificity, stereoselectivity and steady-state kinetic analysis of β-processing domains via diffusible, synthetic substrates† †Electronic supplementary information (ESI) available. See DOI: 10.1039/c5sc01505g Click here for additional data file.

Genetic analysis of polyketide synthase and peptide synthase genes of ‎cyanobacteria as a mining tool for new pharmaceutical compounds

Cyanobacteria are considered a promising source for new ‎pharmaceutical lead compounds and a large number of chemically diverse and ‎bioactive metabolites have been obtained from cyanobacteria. Despite of ‎several worldwide studies on prevalence of NRPSs and PKSs among the ‎cyanobacteria, none of them included Iranian cyanobacteria of Kermanshah ‎province. Therefore, the aim of this study was t...

Detection and Relation of Polyketide Synthase (PKSs) Genes With Antimicrobial Activity in Terrestrial Cyanobacteria of Lavasan

Background and Aims: Cyanobacteria are considered as favorable source for new pharmaceutical compounds. To date, the majority of bioactive metabolites isolated from cyanobacteria are either polyketides (PKSs) or non-ribosomal peptides. Despite of several worldwide studies on prevalence of PKSs, none of them included the terrestrial cyanobacteria of the Lavasan. Therefore, this study aimed to de...

Evidence for an iterative module in chain elongation on the azalomycin polyketide synthase

The assembly-line synthases that produce bacterial polyketide natural products follow a modular paradigm in which each round of chain extension is catalysed by a different set or module of enzymes. Examples of deviation from this paradigm, in which a module catalyses either multiple extensions or none are of interest from both a mechanistic and an evolutionary viewpoint. We present evidence tha...